Despite the devastating impact of vector mosquitoes on human society, we know very little about mosquito development. 15 years ago, I began to use my training as a comparative arthropod developmental biologist to address this gap in our knowledge of mosquito biology. We first developed methods to pursue siRNA-mediated gene silencing to study development of the mosquito nervous system. In recent years, our large-scale efforts to apply RNA interference to mosquito control resulted in the development of yeast interfering RNA larvicides that target multiple species of disease vector mosquitoes. This discovery led to a journey from the bench to the field that has involved a collaborative effort among molecular geneticists, entomologists, field biologists, Ministry of Health employees, bioengineers, ethicists, social scientists, as well as business and technology transfer experts. We are also pursuing several new initiatives, including the extension of RNAi-based yeast technology to adult mosquito control, as well as for the sex separation and mass rearing of male mosquitoes to facilitate population-based mosquito control programs.